control viral vectors  (Addgene inc)

Journal: BMC Molecular and Cell Biology

Article Title: Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells

doi: 10.1186/s12860-023-00494-1

Figure Lengend Snippet: ( A ) The expression level of Emi1 of each group measured by RT-QPCR. ( B ) The bright field view of MDA-MB-231 and SUM149PT cells under a microscope after lentivirus infection. ( C ) Fluorescence images of MDA-MB-231 and SUM149PT cells under a microscope after lentivirus infection. CON: control cells transfected with LV-CON, KD: cells transfected with Emi1-RNAi, NC: cells transfected with LV-GFP virous. All data were expressed as Mean ± SD, *P < 0.05

Article Snippet: The human breast cancer cell strains MDA-MB-231 and SUM149PT were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences, and lentiviral scrambling vector LV-Emi1-ribonucleic interference (RNAi) and negative-control viral vector LV-Emi1-RNAi were obtained from Shanghai Genechem.

Techniques: Expressing, Quantitative RT-PCR, Microscopy, Infection, Fluorescence, Transfection

Journal: BMC Molecular and Cell Biology

Article Title: Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells

doi: 10.1186/s12860-023-00494-1

Figure Lengend Snippet: ( A ) The expression level of Emi1 of each group measured by RT-QPCR. ( B ) Immunoblotting results of Emi1 expression in different groups. ( C ) Statistic graph of Emi1 relative expression. CON: control cells transfected with LV-CON, KD: cells transfected with Emi1-RNAi, NC: cells transfected with LV-GFP virus. All data were expressed as Mean ± SD, ns means no significance, *P < 0.05

Article Snippet: The human breast cancer cell strains MDA-MB-231 and SUM149PT were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences, and lentiviral scrambling vector LV-Emi1-ribonucleic interference (RNAi) and negative-control viral vector LV-Emi1-RNAi were obtained from Shanghai Genechem.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, Virus

Journal: BMC Molecular and Cell Biology

Article Title: Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells

doi: 10.1186/s12860-023-00494-1

Figure Lengend Snippet: ( A ) The statistic graph of numbers of transferred cells in Transwell assay. ( B ) The statistic graph of the changes in the number of cells transferred in the transwell chamber. CON: control cells transfected with LV-CON, KD: cells transfected with Emi1-RNAi, NC: cells transfected with LV-GFP virous. All data were expressed as Mean ± SD, * P < 0.05

Article Snippet: The human breast cancer cell strains MDA-MB-231 and SUM149PT were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences, and lentiviral scrambling vector LV-Emi1-ribonucleic interference (RNAi) and negative-control viral vector LV-Emi1-RNAi were obtained from Shanghai Genechem.

Techniques: Transwell Assay, Transfection

Journal: BMC Molecular and Cell Biology

Article Title: Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells

doi: 10.1186/s12860-023-00494-1

Figure Lengend Snippet: ( A - D ) The statistic graph of proliferation-related genes mRNA level in different groups. ( E - G ) The statistic graph of invasive-related genes mRNA level in different groups. CON: control cells transfected with LV-CON, KD: cells transfected with Emi1-RNAi, All data were expressed as Mean ± SD, *P < 0.05

Article Snippet: The human breast cancer cell strains MDA-MB-231 and SUM149PT were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences, and lentiviral scrambling vector LV-Emi1-ribonucleic interference (RNAi) and negative-control viral vector LV-Emi1-RNAi were obtained from Shanghai Genechem.

Techniques: Transfection